丁酰膽堿酯酶單克隆抗體
規(guī)格：1mg/1ml
英文名： BCHE(1E8)
別名： Cholinesterase; Acylcholine acylhydrolase; Choline esterase II; CHE1; Butyrylcholine esterase; Pseudocholinesterase; butyrylcholinesterase. CHLE_HUMAN.
分子量： 66kDa
儲存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆類型：Polyclonal
亞型：IgG
純化方法：affinity purified by Protein A
**原：Full length native protein purified from human
交叉反應(yīng)：Human,
丁酰膽堿酯酶單克隆抗體細(xì)胞定位：
產(chǎn)品介紹：background: Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008]. Function: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. 丁酰膽堿酯酶單克隆抗體Can degrade neurotoxic organophosphate esters. Subunit: Homotetramer; disulfide-linked. Dimer of dimers. Subcellular Location: Secreted. Tissue Specificity: Detected in blood plasma (at protein level). Present in most cells except erythrocytes. DISEASE: Butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]: Metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the type-B carboxylesterase/lipase family.丁酰膽堿酯酶單克隆抗體 Gene ID: 590 Database links: Entrez Gene: 590 Human Omim: 177400 Human SwissProt: P06276 Human Unigene: 420483 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 血清丁酰膽堿酯酶是由四個亞基組成的糖蛋白，分子量28萬～35萬。作為一類糖蛋白，主要存在于腦、肝、血及膽堿能神經(jīng)末稍的突觸間隙中，主要由肝臟合成，并存在于血清中，生理作用不明，稱為假性膽堿酯酶或丁酰膽堿酯酶[1]Lockridge等采用Edman降解法測定了人血清BChE完整的氨基酸序列，共含有574個氨基酸殘基。BChE氨基酸序列與其他酯酶家族蛋白有很高的同源性。以電鰩AChE的三維結(jié)構(gòu)為模板對人BChE的三維結(jié)構(gòu)進(jìn)行計算機(jī)模擬結(jié)果表明，BChE活性中心，囊袋的底部為Ser198，其兩側(cè)相距一定距離分別是?；Y(jié)合部位和膽堿結(jié)合部位。研究發(fā)現(xiàn)人BChE的氨基酸序列上有Zn2+離子結(jié)合結(jié)構(gòu)。
丁酰膽堿酯酶單克隆抗體產(chǎn)品應(yīng)用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究領(lǐng)域：心血管
儲存條件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
來源： Mouse
外觀： Lyophilized or Liquid