胰島素降解酶抗體

規(guī)格：1mg/1ml

英文名： IDE

別名： BC2; Insulin degrading enzyme; FLJ35968; insulin protease; insulinase; insulysin; Abeta-degrading protease; FLJ35968; Ide; IDE_HUMAN; Insulin-degrading enzyme; OTTHUMP.

分子量： 117kDa

儲存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce

克隆類型：Polyclonal

亞型：IgG

純化方法：affinity purified by Protein A

**原：KLH conjugated synthetic peptide derived from human IDE

交叉反應(yīng)：Human, Mouse, Rat, Chicken, Pig, Cow,

胰島素降解酶抗體細胞定位：細胞膜

產(chǎn)品介紹：background: Insulysin was identified nearly a century ago as an enzyme responsible for the degradation of insulin in cells, although the precise interactions between insulin and insulysin remain elusive. Human insulysin was cloned in 1988, and shown to be a 118 kDa protein that exists primarily as a homodimer, and perhaps also complexed with other molecules. The sequence is well conserved between humans, rats and mice, and the antibody recognizes these species. Insulysin is a metalloproteinase of the clan ME, family M16, which contains an active site HxxEH, a reversal of the canonical HExxH zinc binding motif. Considered a zinc metalloproteinase, the activity of insulysin can be blocked with EDTA or 1-10 phenanthroline. In addition to the active metalloproteinase domain, insulysin contains a second metalloproteinase site which is considered catalytically inactive, and is thought to assist in substrate binding. Insulysin is most closely related to the bacterial proteinase pitrilysin, (the human orthologue of which appears to be MPRP1) and the mammalian proteinsae nardilysin. Generally thought to be a cytoplasmic protein, insulysin has been isolated from many different tissues and 胰島素降解酶抗體cell lines, and can degrade intact insulin, insulin B chain, glucagon, denatured hemoglobin, alpha amyloid protein, TGF alpha and amylin. Recent work implicates insulysin in clearing beta amyloid plaques from the brain, and has generated much interest in Alzheimer’s disease research. The pH optimum for insulysin is basic, pH 8.5, which also distinguishes it from other metalloproteinases. Function: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Subunit: Homodimer. Can form higher oligomers. Interacts (via N-terminus) with varicella-zoster virus (VZV) envelope glycoprotein E (via N-terminus); th胰島素降解酶抗體e membrane-associated isoform may function as an entry receptor for this virus. Subcellular Location: Cytoplasm. Cell membrane. Secreted. Note=Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the peptidase M16 family. Gene ID: 3416 Database links: Entrez Gene: 3416 Human Entrez Gene: 15925 Mouse Entrez Gene: 25700 Rat Omim: 146680 Human SwissProt: P14735 Human SwissProt: Q9JHR7 Mouse SwissProt: P35559 Rat Unigene: 500546 Human Unigene: 28366 Mouse Unigene: 45029 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

胰島素降解酶抗體產(chǎn)品應(yīng)用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

研究領(lǐng)域：心血管  細胞生物  **學(xué)  神經(jīng)生物學(xué)  信號轉(zhuǎn)導(dǎo)  生長因子和**  合成與降解  糖尿病  

儲存條件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

來源： Rabbit

外觀： Lyophilized or Liquid

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