LOCUS Exported 7014 bp ds-DNA circular SYN 29-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBE2R
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7014)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 29, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..7014
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 803..907
/gene="bla"
/label=AmpR promoter
CDS 908..1768
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1939..2527
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3930..4700)
/codon_start=1
/label=KanR/NeoR
/translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG
QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT
FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD
SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
CDS complement(4860..5864)
/codon_start=1
/gene="repB"
/product="RepB replication protein"
/label=repB
/note="from Enterococcus faecalis plasmid pAM-alpha-1"
/db_xref="GI:22652809"
/protein_id="AAN03827.1"
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
misc_feature 6283..6587
/label=P43 promoter
misc_feature 6620..6952
/label=Apr sp+pro
CDS 6983..7000
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
ORIGIN
1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
61 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
121 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
181 ctggtaacgc cgttccgcac cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
241 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
301 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
361 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
421 gccatctcct tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
481 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg atatggtgca ctctcagtac
541 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
601 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
661 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
721 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
901 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
961 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
1021 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
1081 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
1141 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
1201 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
1261 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
1321 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
1381 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
1441 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
1501 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
1561 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
1621 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
1681 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
1741 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
1801 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
1861 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
1921 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
1981 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
2041 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
2101 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
2161 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
2221 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
2281 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
2341 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
2401 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
2461 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
2521 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
2581 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
2641 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
2701 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
2761 cagctgattt cactttttgc attctacaaa ctgcataact catatgtaaa tcgctccttt
2821 ttaggtggca caaatgtgag gcattttcgc tctttccggc aaccacttcc aagtaaagta
2881 taacacacta tactttatat tcataaagtg tgtgctctgc gaggctgtcg gcagtgccga
2941 ccaaaaccat aaaaccttta agacctttct tttttttacg agaaaaaaga aacaaaaaaa
3001 cctgccctct gccacctcag caaagggggg ttttgctctc gtgctcgttt aaaaatcagc
3061 aagggacagg tagtattttt tgagaagatc actcaaaaaa tctccacctt taaacccttg
3121 ccaattttta ttttgtccgt tttgtctagc ttaccgaaag ccagactcag caagaataaa
3181 atttttattg tctttcggtt ttctagtgta acggacaaaa ccactcaaaa taaaaaagat
3241 acaagagagg tctctcgtat cttttattca gcaatcgcgc ccgattgctg aacagattaa
3301 taatagattt tagcttttta tttgttgaaa aaagctaatc aaattgttgt cgggatcaat
3361 tactgcaaag tctcgttcat cccaccactg atcttttaat gatgtattgg ggtgcaaaat
3421 gcccaaaggc ttaatatgtt gatataattc atcaattccc tctacttcaa tgcggcaact
3481 agcagtacca gcaataaacg actccgcacc tgtacaaacc ggtgaatcat tactacgaga
3541 gcgccagctt catcacttgc ctcccataga tgaatccgaa cctcattaca cattagaact
3601 gcgaatccat cttcatggtg aaccaaagtg aaacctagtt tatcgcaata aaaacctata
3661 ctctttttaa tatccccgac tggcaatgcc gggatagact gtaacattct cacgcataaa
3721 atcccctttc attttctaat gtaaatctat taccttatta ttaattcaat tcgctcataa
3781 ttaatccttt ttcttattac gcaaaatggc ccgatttaag cacacccttt attccgttaa
3841 tgcgccatga cagccatgat aattactaat actaggagaa gttaataaat acgtaaccaa
3901 catgattaac aattattaga ggtcatcgtt caaaatggta tgcgttttga cacatccact
3961 atatatccgt gtcgttctgt ccactcctga atcccattcc agaaattctc tagcgattcc
4021 agaagtttct cagagtcgga aagttgacca gacattacga actggcacag atggtcataa
4081 cctgaaggaa gatctgattg cttaactgct tcagttaaga ccgaagcgct cgtcgtataa
4141 cagatgcgat gatgcagacc aatcaacatg gcacctgcca ttgctacctg tacagtcaag
4201 gatggtagaa atgttgtcgg tccttgcaca cgaatattac gccatttgcc tgcatattca
4261 aacagctctt ctacgataag ggcacaaatc gcatcgtgga acgtttgggc ttctaccgat
4321 ttagcagttt gatacacttt ctctaagtat ccacctgaat cataaatcgg caaaatagag
4381 aaaaattgac catgtgtaag cggccaatct gattccacct gagatgcata atctagtaga
4441 atctcttcgc tatcaaaatt cacttccacc ttccactcac cggttgtcca ttcatggctg
4501 aactctgctt cctctgttga catgacacac atcatctcaa tatccgaata gggcccatca
4561 gtctgacgac caagagagcc ataaacacca atagccttaa catcatcccc atatttatcc
4621 aatattcgtt ccttaatttc atgaacaatc ttcattcttt cttctctagt cattattatt
4681 ggtccattca ctattctcat tcccttttca gataatttta gatttgcttt tctaaataag
4741 aatatttgga gagcaccgtt cttattcagc tattaataac tcgtcttcct aagcatcctt
4801 caatcctttt aataacaatt atagcatcta atcttcaaca aactggcccg tttgttgaac
4861 tactctttaa taaaataatt tttccgttcc caattccaca ttgcaataat agaaaatcca
4921 tcttcatcgg ctttttcgtc atcatctgta tgaatcaaat cgccttcttc tgtgtcatca
4981 aggtttaatt ttttatgtat ttcttttaac aaaccaccat aggagattaa ccttttacgg
5041 tgtaaacctt cctccaaatc agacaaacgt ttcaaattct tttcttcatc atcggtcata
5101 aaatccgtat cctttacagg atattttgca gtttcgtcaa ttgccgattg tatatccgat
5161 ttatatttat ttttcggtcg aatcatttga acttttacat ttggatcata gtctaatttc
5221 attgcctttt tccaaaattg aatccattgt ttttgattca cgtagttttc tgtattctta
5281 aaataagttg gttccacaca taccaataca tgcatgtgct gattataaga attatcttta
5341 ttatttattg tcacttccgt tgcacgcata aaaccaacaa gatttttatt aattttttta
5401 tattgcatca ttcggcgaaa tccttgagcc atatctgaca aactcttatt taattcttcg
5461 ccatcataaa catttttaac tgttaatgtg agaaacaacc aacgaactgt tggcttttgt
5521 ttaataactt cagcaacaac cttttgtgac tgaatgccat gtttcattgc tctcctccag
5581 ttgcacattg gacaaagcct ggatttacaa aaccacactc gatacaactt tctttcgcct
5641 gtttcacgat tttgtttata ctctaatatt tcagcacaat cttttactct ttcagccttt
5701 ttaaattcaa gaatatgcag aagttcaaag taatcaacat tagcgatttt cttttctctc
5761 catggtctca cttttccact ttttgtcttg tccactaaaa cccttgattt ttcatctgaa
5821 taaatgctac tattaggaca cataatatta aaagaaaccc ccatctattt agttatttgt
5881 ttagtcactt ataactttaa cagatggggt ttttctgtgc aaccaatttt aagggttttc
5941 aatactttaa aacacataca taccaacact tcaacgcacc tttcagcaac taaaataaaa
6001 atgacgttat ttctatatgt atcaagataa gaaagaacaa gttcaaaacc atcaaaaaaa
6061 gacacctttt caggtgcttt ttttatttta taaactcatt ccctgatctc gacttcgttc
6121 tttttttacc tctcggttat gagttagttc aaattcgttc tttttaggtt ctaaatcgtg
6181 tttttcttgg aattgtgctg ttttatcctt taccttgtct acaaacccct taaaaacgtt
6241 tttaaaggct tttaagccgt ctgtacgttc cttaaggaat tcgagctcag ctttattgag
6301 tggatgatta tattcctttt gataggtggt atgttttcgc ttgaactttt aaatacagcc
6361 attgaacata cggttgattt aataactgac aaacatcacc ctcttgctaa agcggccaag
6421 gacgctgccg ccggggctgt ttgcgttttt gccgtgattt cgtgtattat tggtttactt
6481 atttttttgc caaagctgta atggctgaaa attcttacat ttattttaca tttttagaaa
6541 tgggcgtgaa aaaaagcgcg cgattatgta aaatataaag tgatagcggt accattatag
6601 gtaagagagg aatgtacaca tgaagaaacc gttggggaaa attgtcgcaa gcaccgcact
6661 actcatttct gttgctttta gttcatcgat cgcatcggct gctgaagaag caaaagaaaa
6721 atatttaatt ggctttaatg agcaggaagc tgtcagtgag tttgtagaac aagtagaggc
6781 aaatgacgag gtcgccattc tctctgagga agaggaagtc gaaattgaat tgcttcatga
6841 atttgaaacg attcctgttt tatccgttga gttaagccca gaagatgtgg acgcgcttga
6901 actcgatcca gcgatttctt atattgaaga ggatgcagaa gtaacgacaa tgggatcctc
6961 tagagtcgac ctgcagctcg agcaccacca ccaccaccac aagcttgtat tcta
//