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pAC5.1b-EGFP-2

pAC5.1b-EGFP-2
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  • 產(chǎn)品名稱：pAC5.1b-EGFP-2
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  • 產(chǎn)品展商：XYbscience
  • 產(chǎn)品文檔：無相關(guān)文檔
簡單介紹
pAC5.1b-EGFP-2質(zhì)粒是一個昆蟲細(xì)胞組成型表達(dá)載體，Ac5啟動子驅(qū)動EGFP標(biāo)簽、目的基因、Myc標(biāo)簽和6×HIS標(biāo)簽一起融合表達(dá)，pAC5.1b-EGFP-2可以與篩選載體pCoHygro或者pCoBlast共轉(zhuǎn)染，建立穩(wěn)表達(dá)細(xì)胞系。
產(chǎn)品描述
 

pAC5.1b-EGFP-2

  • 產(chǎn)品信息
  • 基本信息
  • 質(zhì)粒簡介
  • 質(zhì)粒圖譜
  • 質(zhì)粒序列

產(chǎn)品信息

產(chǎn)品貨號 產(chǎn)品名稱 產(chǎn)品規(guī)格 優(yōu)惠價
XY1399 pAC5.1b-EGFP-2

1μg

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使用說明

信裕質(zhì)粒平臺的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過嚴(yán)格的多重驗(yàn)證，如存在質(zhì)量問題，請?jiān)谑盏疆a(chǎn)品的三個月內(nèi)通知我司。收到質(zhì)粒后請短暫離心，加入20μl ddH2O溶解質(zhì)粒，取2μl轉(zhuǎn)化至對應(yīng)感受態(tài)中，挑取單克隆重新提取質(zhì)粒后使用。如需獲取其他詳細(xì)信息請登錄我司官網(wǎng)查詢。

基本信息

啟動子: Ac5 promoter
復(fù)制子: pUC ori
終止子: SV40 poly(A) signal
質(zhì)粒分類: 昆蟲系列質(zhì)粒；昆蟲游離質(zhì)粒；昆蟲胞內(nèi)質(zhì)粒
質(zhì)粒大小: 6118bp
質(zhì)粒標(biāo)簽: N-EGFP，C-V5，C-6×His
原核抗性: 氨芐青霉素Amp
篩選標(biāo)記: 無
克隆菌株: DH5α等大腸桿菌
培養(yǎng)條件: 37℃，有氧，LB
表達(dá)宿主: 果蠅細(xì)胞系S2
誘導(dǎo)方式: 無需誘導(dǎo)，組成型表達(dá)
5'測序引物: Ac5-F(ACACAAAGCCGCTCCATCAG)
3'測序引物: BGH-R(TAGAAGGCACAGTCGAGG)
備注: 非病毒

質(zhì)粒簡介

          pAC5.1b-EGFP-2質(zhì)粒是一個昆蟲細(xì)胞組成型表達(dá)載體，Ac5啟動子驅(qū)動EGFP標(biāo)簽、目的基因、Myc標(biāo)簽和6×HIS標(biāo)簽一起融合表達(dá)，可以與篩選載體pCoHygro或者pCoBlast共轉(zhuǎn)染，建立穩(wěn)表達(dá)細(xì)胞系。
      pAC5.1b-EGFP② is a 6kb expression vector designed for use with the Drosophila Constitutive Expression System available from Invitrogen. Upon transfection, the vector allows transient expression of your protein of interest in Drosophila cells. When cotransfected with the selection vector, pCoHygro or pCoBlast, pAC5.1b-EGFP allows selection of stable cell lines exhibiting constitutive expression of the protein of interest.
                ? The Drosophila actin 5C (Ac5) promoter for high-level, constitutive expression of the gene of interest in S2 cells (Chung and Keller, 1990).
                ? Multiple cloning site to facilitate cloning the gene of interest.
                ? C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for detection and purification of your protein of interest (if desired).
                ? Three reading frames to facilitate in-frame cloning with the C-terminal peptide.
                ? N-terminal EGFP tag for better detection.
                ? Ampicillin resistance gene for selection of transformants in E. coli.

質(zhì)粒圖譜



質(zhì)粒序列

LOCUS       Exported                6118 bp ds-DNA     circular SYN 25-AUG-2017
DEFINITION  synthetic circular DNA
KEYWORDS    pAC5.1b-EGFP②
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6118)
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2017 from MiaoLingPlasmid
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     primer_bind     2525..2544
                     /label=AC5-F
     CDS             2617..3333
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    3336..3402
                     /label=MCS
     CDS             3403..3444
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3454..3471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3544..3678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3622..3641)
                     /label=Sv40-polyA-R
     rep_origin      complement(4300..4888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5059..5919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5920..6024)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctactag tccagtgtgg tggaatttcg agctttatgg tgagcaaggg cgaggagctg
     2641 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     2701 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     2761 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     2821 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     2881 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2941 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3001 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     3061 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     3121 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     3181 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     3241 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3301 gggatcactc tcggcatgga cgagctgtac aagccaagct tgaattctgc agatatccag
     3361 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     3421 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa
     3481 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc
     3541 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3601 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3661 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3721 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     3781 gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     3841 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     3901 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat
     3961 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac
     4021 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga
     4081 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
     4141 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
     4201 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagccagca
     4261 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4441 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     4501 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4561 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4621 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4681 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4741 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4801 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4861 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4921 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4981 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5041 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5101 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5161 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5221 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5281 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5341 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc
     5401 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5461 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5521 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5581 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5641 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     5701 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5761 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5821 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5881 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5941 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6001 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     6061 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt
//
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