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- 產(chǎn)品名稱：Recombinant Protein G, His-tag
- 產(chǎn)品型號(hào)：
- 產(chǎn)品展商：XYbscience
- 產(chǎn)品文檔：無相關(guān)文檔
簡(jiǎn)單介紹
Recombinant Protein G, His-tag由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。Recombinant Protein G, His-tag而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)???使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
產(chǎn)品描述
Recombinant Protein G, His-tag
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY5030-1g
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Recombinant
Protein G, His-tag
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1g
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24768.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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－
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－
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－
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E. coli
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210aa
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29kDa
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His
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About this
Protein
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Name
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Recombinant
Protein G, His-tag (Recombinant Protein G, His-tag; Protein G, His-tag)；重組蛋白G，His標(biāo)簽
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Synonyms
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N/A
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Purity
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>97% by
SDS-PAGE and RP-HPLC analyses
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Biological
Activity
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The
recombinant Protein G is a genetically engineered protein containing 3
IgG-binding regions of protein G. Cell wall binding region, cell membrane
binding region and albumin binding region have been removed from the
recombinant Protein G to ensure the maximum specific IgG binding. The
recombinant Protein G is ideal for purification of polyclonal or monoclonal
IgG antibodies. Protein G binds to various human, mouse and rat IgG
subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat
IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house and
rabbit.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from additive free solution.
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Endotoxin
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Less than
0.1EU/μg of Protein G, His-tag as determined by LAL method.
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Reconstitution
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Dissolve in
distilled water or saline.
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Category
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Protein G
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Background
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Protein G is a
bacterial protein derived from the cell wall of certain strains of
b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of
various classes and subclasses of immunoglobulins from a variety of species.
Protein G binds to all IgG subclasses from human, mouse and rat species. It
also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
Protein G binds preferentially to the Fc portion of IgG, but can also bind to
the Fab region, making it useful for purification of F(ab') fragments of IgG.
Due to it's affinity for the Fc region of many mammalian immunoglobulins,
protein G is considered a universal reagent in biochemistry and immunology.
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Amino Acid
Sequence
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MGTYKLILNG KTLKGETTTE AVDAATAEKV
FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTE AVDAATAEKV
FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTK AVDAETAEKA
FKQYANDNGV DGVWTYDDAT KTFTVTEKLA AALEHHHHHH
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包裝清單:
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY5030-1mg
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Recombinant
Protein G, His-tag
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1mg
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XY5030-10mg
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Recombinant
Protein G, His-tag
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10mg
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XY5030-1g
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Recombinant
Protein G, His-tag
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1g
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-
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說明書
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1份
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保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。
注意事項(xiàng):
由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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