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- 產(chǎn)品名稱：Recombinant Human TNF-α/TNFSF2, His-tag
- 產(chǎn)品型號：
- 產(chǎn)品展商：XYbscience
- 產(chǎn)品文檔：無相關(guān)文檔
簡單介紹
Recombinant Human TNF-α/TNFSF2, His-tag由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA???)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點，使細胞因子或重組蛋白不會粘附于管壁。Recombinant Human TNF-α/TNFSF2, His-tag所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
產(chǎn)品描述
Recombinant Human TNF-α/TNFSF2, His-tag
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY322-1mg
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Recombinant
Human TNF-α/TNFSF2, His-tag
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1mg
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9237.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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7124
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P01375
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E. coli
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164aa
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18.3kDa
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His
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About this
protein
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Name
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Recombinant
Human TNF-α/TNFSF2, His-tag (Recombinant Human Tumor Necrosis
Factor-α/TNFSF2, His-tag; rHuTNF-α/TNFSF2, His-tag)；重組人腫瘤壞死因子α/TNFSF2，His標(biāo)簽
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Synonyms
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Cachetin; DIF;
TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF,
monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived;
TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor
(TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis
factor ligand superfamily member 2; tumor necrosis factor-alpha
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Purity
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>97% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cytotoxicity assay using murine L929 cells is less than 0.05ng/ml,
corresponding to a specific activity of >2.0×107IU/mg
in the presence of actinomycin D.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.0.
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Endotoxin
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Less than 1EU/μg of rHuTNF-α/TNFSF2, His-tag as
determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should
be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Tumor necrosis
factor alpha (TNF-α), also called cachectin, is the best-know member of
the TNF-family, which can cause cell death. This protein is produced by
neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells,
astrocytes endothelial cells, smooth muscle cells and some transformed cells.
TNF-α occurs as a secreted, soluble form and as a
membrane-anchored form, both of which are biologically active. The
naturally-occurring form of TNF-α is glycosylated,
but non-glycosylated recombinant TNF-α has comparable
biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and
cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the
presence of one or both of these receptor types.
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Amino Acid
Sequence
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MHHHHHHVRS SSRTPSDKPV AHVVANPQAE
GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL
LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
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包裝清單:
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY322-10μg
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Recombinant
Human TNF-α/TNFSF2, His-tag
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10μg
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XY322-50μg
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Recombinant
Human TNF-α/TNFSF2, His-tag
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50μg
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XY322-100μg
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Recombinant
Human TNF-α/TNFSF2, His-tag
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100μg
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XY322-1mg
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Recombinant
Human TNF-α/TNFSF2, His-tag
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1mg
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-
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說明書
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1份
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保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應(yīng)濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。
注意事項:
由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
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