Recombinant Human NT-4
產(chǎn)品編號
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價格
XY5678-1mg
1mg
26773.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
4909
P34130
E. coli
262aa
28.1kDa
－
About this Antibody
Name
Recombinant Human NT-4 (Recombinant Human Neurotrophin-4; rHuNT-4)；重組人神經(jīng)營養(yǎng)因子4
Synonyms
NT-5; NTF4; NTF5; GLC10; GLC1O; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 4; neurotrophin 5 (neurotrophin 4/5); neurotrophin-4; Neurotrophin-5; neurotrophin-5; Neutrophic factor 4; NT4; NT-4; NT-4/5; NT-5; NTF5NT5
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50ng/ml, corresponding to a specific activity of >2.0×104IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH5.5.
Endotoxin
Less than 1EU/μg of rHuNT-4 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
NT-4 also named as NT-5 is a neuronal and epithelial grow factor belongs to thengF-beta family. The NT-4 precursor is consisted of a 24a.a. signal peptide, a 56a.a. propertied and 130a.a. NT-4. The mature protein has six Cys amino acid residues and has the relative structure with NT-3, BDNF (sharing about 48%-52% sequence identity). Additionally, it shares 91% and 95%a.a. sequence identity with mouse and rat NT-4. NT-4 is mainly expressed in prostate and has low level thymus, placenta, and skeletal muscle. It can binding with the LNGFR and trkB receptors and plays a crucial role in the regulation of survival and the maintenance of peripheral sensory sympathetic neurons. Defect of NT-4 may cause primary open angle glaucoma type 1O.
Amino Acid Sequence
MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A
包裝清單:
包裝
XY5678-2μg
2μg
XY5678-10μg
10μg
XY5678-100μg
100μg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應(yīng)濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。 注意事項: 由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。 本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請穿實驗服并戴一次性手套操作。