Recombinant Murine IL-3
產(chǎn)品編號
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價格
XY5912-1mg
1mg
15444.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
16187
P01586
E. coli
134aa
14.8kDa
-
About this Antibody
Name
Recombinant Murine IL-3 (Recombinant Murine Interleukin-3; rMuIL-3);Recombinant Mouse IL-3 ; 重組小鼠白細胞介素3
Synonyms
Hematopoietic growth factor; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of >2×107IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in PBS, pH7.4.
Endotoxin
Less than 1EU/μg of rMuIL-3 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition, it exerts its biological activities through binding to interleukin-3 receptors included α and β subunits. The Mouse IL-3 is different from human IL-3 and contains 140 amino acids residues. Specifically, mouse and human IL-3 share low homology and have not cross species activity.
Amino Acid Sequence
DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC
包裝清單:
包裝
XY5912-2μg
2μg
XY5912-10μg
10μg
XY5912-100μg
100μg
-
說明書
1份
保存條件: -20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會引起部分失活,所以**配制成相應濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液),須分裝后-20℃或更低溫度凍存,以避免反復凍融。 注意事項: 由于有些塑料管壁對某些蛋白有較強的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結(jié)合位點,使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。 本產(chǎn)品**于專業(yè)人員的科學研究用,不得用于臨床診斷或**,不得用于食品或藥品,不得存放于普通住宅內(nèi)。 為了您的**和健康,請穿實驗服并戴一次性手套操作。