Recombinant Murine MANF
產(chǎn)品編號
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價格
XY6168-1mg
1mg
15611.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Mouse
74840
Q3TMX5
E. coli
158aa
18.2kDa
－
About this Antibody
Name
Recombinant Murine MANF (Recombinant Murine Mesencephalic AstrocyteDerived Neurotrophic Factor; rMuMANF)；Recombinant Mouse MANF ; 重組小鼠中腦星形膠質(zhì)細胞源性神經(jīng)營養(yǎng)因子
Synonyms
ARMET; ARP; Arginine-Rich Protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 10μg/ml, corresponding to a specific activity of >100IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rMuMANF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into workng aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parknson disease. Mature murine MANF is 99%, 98% and 95%a.a. identical to mature rat, human and bovine MANF respectively.
Amino Acid Sequence
LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIEEELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDNQ IDLSTVDLKK LRVKELKKIL DDWGEMCKGC AEKSDYIRKNELMPKYAPK AASARTDL
包裝清單:
包裝
XY6168-5μg
5μg
XY6168-25μg
25μg
XY6168-100μg
100μg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應(yīng)濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。 注意事項: 由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。 本產(chǎn)品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請穿實驗服并戴一次性手套操作。