Recombinant Rat IFN-γ
產(chǎn)品編號(hào)
產(chǎn)品名稱(chēng)
產(chǎn)品包裝
產(chǎn)品價(jià)格
XY6398-1mg
1mg
5665.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Rat
25712
P01581
E. coli
134aa
15.5kDa
－
About this Protein
Name
Recombinant Rat IFN-γ (Recombinant Rat Interferon-γ; rRtIFN-γ)；重組大鼠干擾素γ
Synonyms
IFNG; IFG; IFI; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 1X PBS, pH7.4, 1mM DTT, 5% Trehalose and 0.05% Tween-80.
Endotoxin
Less than 0.1EU/μg of rRtIFN-γ as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a cytokine produced primarily by Tlymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. It shares high sequence indentity with murine IFN-γ (86%). IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-γ augments TH1 cell expansion and may be required for TH1 cell differentiation.
Amino Acid Sequence
QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC
包裝清單:
包裝
XY6398-20μg
20μg
XY6398-100μg
100μg
-
說(shuō)明書(shū)
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。 注意事項(xiàng): 由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。 本產(chǎn)品**于專(zhuān)業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。