Recombinant Rat Midkine
產(chǎn)品編號(hào)
產(chǎn)品名稱(chēng)
產(chǎn)品包裝
產(chǎn)品價(jià)格
XY6425-1mg
1mg
15611.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Rat
81517
Q9R1S9
E. coli
120aa
13.2kDa
-
About this Antibody
Name
Recombinant Rat Midkine (Recombinant Rat Midkine; rRtMidkine);重組大鼠中期因子
Synonyms
ARAP; MDK; MEK; MK1; NEGF2; Amphiregulin-associated protein; Midgestation and kidney protein; midkine; midkine (neurite growth-promoting factor 2); MK1; MKARAP; NEGF2FLJ27379; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin
Less than 0.1EU/μg of rRtMidkine as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family and it is encoded by the MDK gene. Midkine includes five intrachain disulfide bonds which held two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.2kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Mature rat midkine shares 99% and 91%a.a. sequence identity with human and murine midkine, respectively.
Amino Acid Sequence
VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD
包裝清單:
包裝
XY6425-5μg
5μg
XY6425-20μg
20μg
XY6425-100μg
100μg
-
說(shuō)明書(shū)
1份
保存條件: -20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活,所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液),須分裝后-20℃或更低溫度凍存,以避免反復(fù)凍融。 注意事項(xiàng): 由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn),使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。 本產(chǎn)品**于專(zhuān)業(yè)人員的科學(xué)研究用,不得用于臨床診斷或**,不得用于食品或藥品,不得存放于普通住宅內(nèi)。 為了您的**和健康,請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。