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- 產(chǎn)品名稱：Recombinant Human ME2, His-tag
- 產(chǎn)品型號(hào)：
- 產(chǎn)品展商：XYbscience
- 產(chǎn)品文檔：無相關(guān)文檔
簡(jiǎn)單介紹
Recombinant Human ME2, His-tag由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。Recombinant Human ME2, His-tag所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
產(chǎn)品描述
Recombinant Human ME2, His-tag
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY7014-1mg
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Recombinant
Human ME2, His-tag
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1mg
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14568.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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4200
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P23368
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E. coli
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573aa
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64.4kDa
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His
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About this
Antibody
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Name
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Recombinant
Human ME2, His-tag (Recombinant Human Malic Enzyme 2, His-tag; rHuME2,
His-tag)；重組人蘋果酸酶2，His標(biāo)簽
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Synonyms
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NAD-dependent
malic enzyme, mitochondrial; NAD-ME; Malic enzyme 2; ME2
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Malic Enzyme
activity was assayed spectrophotometrically at 340nm as described in Mandela
and Sauer (1975). The standard reaction mixture contained 50mM Tris-HCl, 3mM
MnCl2, 5mM malate, 0.12mM NADP+, 2.5mM fumarate. Assay
was performed in a Beckman spectrophotometer. The Km value is 1.5±0.6mM.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rHuME2, His-tag as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Enzymes
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Background
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NAD-dependent
malic enzyme (ME2), mitochondrial is a protein that in humans is encoded by
the ME2 gene. This gene encodes a mitochondrial NAD-dependent malic enzyme, a
homotetrameric protein, which catalyzes the oxidative decarboxylation of
malate to pyruvate. Three different isoforms of ME are known to be in mammalian
tissues: a strictly cytosolic NADP+-dependent enzyme, an NADP+-dependent
mitochondriail isoform, and a mitochondrial isoenzyme that is able to use
both NAD+ and NADP+ but is more effective with NAD+.
The mammalian isoforms size is about 62-64kDa. A native size of 240,000 Da
proposes a tetrameric structure for the active enzyme.
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Amino Acid
Sequence
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MLHIKEKGKP LMLNPRTNKG MAFTLQERQM
LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG
LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY
TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RNTLIQFEDF
GNHNAFRFLR KYREKYCTFN DDIQGTAAVA LAGLLAAQKV ISKPISEHKI LFLGAGEAAL GIANLIVMSM
VENGLSEQEA QKKIWMFDKY GLLVKGRKAK IDSYQEPFTH SAPESIPDTF EDAVNILKPS TIIGVAGAGR
LFTPDVIRAM ASINERPVIF ALSNPTAQAE CTAEEAYTLT EGRCLFASGS PFGPVKLTDG RVFTPGQGNN
VYIFPGVALA VILCNTRHIS DSVFLEAAKA LTSQLTDEEL AQGRLYPPLA NIQEVSINIA IKVTEYLYAN
KMAFRYPEPE DKAKYVKERT WRSEYDSLLP DVYEWPESAS SPPVITEHHH HHH
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包裝清單:
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY7014-5μg
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Recombinant
Human ME2, His-tag
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5μg
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XY7014-25μg
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Recombinant
Human ME2, His-tag
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25μg
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XY7014-100μg
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Recombinant
Human ME2, His-tag
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100μg
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XY7014-1mg
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Recombinant
Human ME2, His-tag
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1mg
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-
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說明書
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1份
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保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。
注意事項(xiàng):
由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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