Recombinant Bovine EK
產(chǎn)品編號(hào)
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價(jià)格
XY7059-1000IU
1000IU
2662.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Bovine
282009
P98072
E. coli
235aa
28kDa
－
About this Protein
Name
Recombinant Bovine EK (Recombinant Bovine Enterokinase Light Chain; rBoEKL)；重組牛輕鏈腸激酶
Synonyms
Enterokinase; ENTK; PRSS7; TMPRSS15; EC 3.4.21; EC 3.4.21.9; Enterokinase; ENTKenterokinase; MGC133046; protease, serine, 7 (enterokinase); PRSS7enteropeptidase; Serine protease 7; Transmembrane protease serine 15; transmembrane protease, serine 15
Purity
N/A
Biological Activity
Physical Appearance
Sterile liquid.
Formulation
50mM Tris-HCl, pH8.0, 0.5M NaCl and 50% glycerol.
Endotoxin
Less than 1EU/μg of rBoEKL as determined by LAL method.
Reconstitution
Category
Enzymes
Background
Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfidelinked 82–140kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62kDa light chain which contains the catalytic subunit. Additionally, both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue. rEK was report to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications.
Amino Acid Sequence
包裝清單:
包裝
XY7059-100IU
100IU
XY7059-250IU
250IU
-
說(shuō)明書(shū)
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。 注意事項(xiàng): 由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。 本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。