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- 產品名稱：Recombinant Bovine EK, His-tag
- 產品型號：
- 產品展商：XYbscience
- 產品文檔：無相關文檔
簡單介紹
Recombinant Bovine EK, His-tag由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點，使細胞因子或重組蛋白不會粘附于管壁。Recombinant Bovine EK, His-tag所以一定要使用產品簡介中Reconstitution一欄的信息配制儲存液。
產品描述
Recombinant Bovine EK, His-tag
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產品編號
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產品名稱
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產品包裝
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產品價格
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XY7064-1000IU
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Recombinant
Bovine EK, His-tag
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1000IU
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2662.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Bovine
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282009
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P98072
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E. coli
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241aa
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28kDa
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His
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About this
Protein
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Name
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Recombinant
Bovine EK, His-tag (Recombinant Bovine Enterokinase Light Chain, His-tag;
rBoEK, His-tag)；重組牛輕鏈腸激酶，His標簽
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Synonyms
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Enterokinase;
ENTK; PRSS7; TMPRSS15; EC 3.4.21; EC 3.4.21.9; Enterokinase;
ENTKenterokinase; MGC133046; protease, serine, 7 (enterokinase);
PRSS7enteropeptidase; Serine protease 7; Transmembrane protease serine 15;
transmembrane protease, serine 15
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Purity
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N/A
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Biological
Activity
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N/A
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Physical
Appearance
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Sterile
liquid.
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Formulation
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50mM Tris-HCl,
pH8.0, 0.5M NaCl and 50% glycerol.
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Endotoxin
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Less than 1EU/μg of rBoEKL, His-tag as determined by LAL method.
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Reconstitution
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N/A
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Category
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Enzymes
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Background
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Enterokinase
(EK) is an amino protease existing in duodenum of mammal and is involved in
digestion. It consists of a disulfidelinked 82-140kDa heavy chain which
anchors enterokinase in the intestinal brush border membrane and a 35-62kDa
light chain which contains the catalytic subunit. Additionally, both of the
chains are derived from a single precursor that is cleaved by a trypsin-like
protease. EK can specially recognize the amino acid sequence DDDDK, and
digest the peptide bond after the lysine residue. rEK was report to be more
effective than nature EK in cleaving recombinant proteins. Furthermore, the
light chain possesses the whole enzyme activity of EK. rBoEK has the highest
activity than EK of other species and is used wildly in biochemical applications.
rBoEK with 6X His-tag binds with Ni2+ affinity
chromatography and was designed for removing from digestion system.
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Amino Acid
Sequence
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N/A
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包裝清單:
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產品編號
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產品名稱
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包裝
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XY7064-100IU
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Recombinant
Bovine EK, His-tag
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100IU
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XY7064-250IU
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Recombinant
Bovine EK, His-tag
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250IU
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XY7064-1000IU
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Recombinant
Bovine EK, His-tag
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1000IU
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-
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說明書
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1份
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保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應濃度的儲存液后(請根據(jù)產品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復凍融。
注意事項:
由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產品簡介中Reconstitution一欄的信息配制儲存液。
本產品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
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