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- 產(chǎn)品名稱：Recombinant Human UBE2I, His-tag
- 產(chǎn)品型號：
- 產(chǎn)品展商：XYbscience
- 產(chǎn)品文檔：無相關(guān)文檔
簡單介紹
Recombinant Human UBE2I, His-tag由于有些塑料管壁對某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)???因子或重組蛋白不會粘附于管壁。Recombinant Human UBE2I, His-tag所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
產(chǎn)品描述
Recombinant Human UBE2I, His-tag
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY7113-1mg
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Recombinant
Human UBE2I, His-tag
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1mg
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12354.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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7329
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P63279
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E. coli
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158aa
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19.5kDa
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His
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About this
Antibody
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Name
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Recombinant
Human UBE2I, His-tag (Recombinant Human SUMO-Conjugating Enzyme UBC9,
His-tag; rHuUBE2I, His-tag)；重組人泛素結(jié)合酶E2I，His標(biāo)簽
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Synonyms
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p18;
SUMO-protein Ligase; Ubc9; UBCE9; C358B7.1; EC 6.3.2; EC 6.3.2.-; P18; p18;
SUMO-1-protein ligase; SUMO-protein ligase; UBC9SUMO-conjugating enzyme UBC9;
UBCE9; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; ubiquitin
conjugating enzyme 9; Ubiquitin-conjugating enzyme E2 I;
ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating
enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme UbcE2A;
ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase
E2I; Ubiquitin-protein ligase I
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Data is not
available.
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Physical
Appearance
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Sterile
Colorless liquid.
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Formulation
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A 0.2μm filtered concentrated solution in 50mM HEPES, pH7.6, with 125mM
NaCl, 10% Glycerol, 1mM DTT.
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Endotoxin
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Less than 1EU/μg of rHuUBC9, His-tag as determined by LAL method.
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Reconstitution
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N/A
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Category
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Ubiquitins
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Background
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SUMO-Conjugating
Enzyme UBC9 belongs to the ubiquitin-conjugating enzyme family and is encoded
by the UBE2I gene in humans. The ubiquitin-conjugating enzymes, also known as
E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the
second step in the ubiquitination reaction. In this reaction, E1 activates
the ubiquitin by covalently attaching the molecule to its active site
cysteine residue. The activated ubiquitin is then transferred to an E2
cysteine and then the E2 molecule binds E3 via a structurally conserved
binding region. The UBC9 accepts the ubiquitin-like proteins SUMO1-4 from the
UBLE1AUBLE1B E1 complex and catalyzes their covalent attachment to other
proteins with the help of an E3 ligase such as RANBP2 or CBX4. Additionally,
It takes parts in catalysis the formation of poly-SUMO chains, sumoylation of
FOXL2 and KAT5, and the segregation of nuclear architecture and chromosome.
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Amino Acid
Sequence
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MHHHHHHAMG TLNMSGIALS RLAQERKAWR
KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY
PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP
S
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包裝清單:
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY7113-10μg
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Recombinant
Human UBE2I, His-tag
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10μg
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XY7113-50μg
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Recombinant
Human UBE2I, His-tag
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50μg
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XY7113-100μg
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Recombinant
Human UBE2I, His-tag
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100μg
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XY7113-1mg
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Recombinant
Human UBE2I, His-tag
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1mg
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-
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說明書
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1份
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保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應(yīng)濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。
注意事項(xiàng):
由于有些塑料管壁對某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。
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