- 如果您對(duì)該產(chǎn)品感興趣的話，可以
- 產(chǎn)品名稱：Recombinant Human UBE2C, His-tag
- 產(chǎn)品型號(hào)：
- 產(chǎn)品展商：XYbscience
- 產(chǎn)品文檔：無(wú)相關(guān)文檔
簡(jiǎn)單介紹
Recombinant Human UBE2C, His-tag由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)???因子或重組蛋白不會(huì)粘附于管壁。Recombinant Human UBE2C, His-tag所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
產(chǎn)品描述
Recombinant Human UBE2C, His-tag
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY7118-1mg
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Recombinant
Human UBE2C, His-tag
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1mg
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12354.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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11065
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O00762
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E. coli
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191aa
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21.1kDa
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His
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About this
Antibody
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Name
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Recombinant
Human UBE2C, His-tag (Recombinant Human Ubiquitin Conjugating Enzyme E2 C,
His-tag; rHuUBE2C, His-tag)；重組人泛素結(jié)合酶E2C，His標(biāo)簽
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Synonyms
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UBE2C;
cyclin-selective ubiquitin carrier protein; dJ447F3.2; EC 6.3.2.19; UbcH10;
UBCH10mitotic-specific ubiquitin-conjugating enzyme; Ubiquitin carrier
protein C; ubiquitin carrier protein E2-C; ubiquitin-conjugating enzyme E2 C;
ubiquitin-conjugating enzyme E2C; Ubiquitin-protein ligase C
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Data is not
available.
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Physical
Appearance
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Sterile
Colorless liquid.
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Formulation
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A 0.2μm filtered concentrated solution in 50mM HEPES, pH8.0, with 125mM
NaCl, 10% Glycerol, 5% Trehalose, 1mM DTT.
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Endotoxin
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Less than 1EU/μg of rHuUBE2C, His-tag as determined by LAL method.
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Reconstitution
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N/A
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Category
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Ubiquitins
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Background
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Ubiquitin
Conjugating Enzyme E2 C belongs to the ubiquitin-conjugating enzyme family
and is encoded by the UBE2C gene in humans. The ubiquitin-conjugating
enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier
enzymes, take part in the second step in the ubiquitination reaction. In this
reaction, E1 activates the ubiquitin by covalently attaching the molecule to
its active site cysteine residue. The activated ubiquitin is then transferred
to an E2 cysteine and then the E2 molecule binds E3 via a structurally
conserved binding region. The UBE2C catalyzes the destruction of cyclins A
and B in conjunction with the anaphase-promoting complex, and therefore,
plays an important role in the control of the cell exit from mitosis. This
activity is essential at the end of mitosis for the inactivation of their
partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In
addition, UBCH10 bears homology to yeast PAS2, a gene that is essential for
biogenesis of peroxisomes. UBCH10 is useful for in vitro ubiquitinylation
reactions.
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Amino Acid
Sequence
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MHHHHHHAMG IRMASQNRDP AATSVAAARK
GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG
YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN
PTAFKKYLQE TYSKQVTSQE P
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包裝清單:
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY7118-10μg
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Recombinant
Human UBE2C, His-tag
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10μg
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XY7118-50μg
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Recombinant
Human UBE2C, His-tag
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50μg
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XY7118-100μg
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Recombinant
Human UBE2C, His-tag
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100μg
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XY7118-1mg
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Recombinant
Human UBE2C, His-tag
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1mg
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-
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說明書
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1份
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保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。
注意事項(xiàng):
由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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