Recombinant Human MIC-B
產(chǎn)品編號
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價格
XY7149-1mg
1mg
12354.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
4277
Q29980
E. coli
287aa
32.8kDa
－
About this Antibody
Name
Recombinant Human MIC-B (Recombinant Human MHC Class I PolypeptideRelated Sequence B; rHuMIC-B)；重組人MIC類多肽相關(guān)序列B
Synonyms
MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence B; MIC-B; PERB11.2MHC class I-like molecule PERB11.2-IMX; stress inducible class I homolog
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The specific activity is determined by binding MICB antibody in ELISA.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris, 150mM NaCl, pH8.0.
Endotoxin
Less than 1EU/μg of rHuMIC-B as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
MIC-B (MHC class I chain-related gene B) is a single-pass type I member protein. It is widely expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In addition to this, it is produced by hepatocellular carcinomas, which is only in tumor cells but not in surrounding non-cancerous tissue and can be induced by bacterial and viral infections. MIC-B shares 85% amino acid identity with MIC-A and they are distantly related to the MHC class I proteins. Because they possess three extracellular Ig-like domains, but unlike classical MHC class I molecules, they do not form a heterodimer with beta2 microglobulin, but bind as a monomer to a KLRK1/NKG2D that is an activating receptor expressed on NK cells, NKT cells, γδ T cells, and CD8+ αβ T cells. Recognition of MIC-B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MIC-B recognition plays an important role in tumor surveillance, viral infections, and autoimmune diseases.
Amino Acid Sequence
AEPHSLRYNL MVLSQDESVQ SGFLAEGHLD GQPFLRYDRQ KRRAKPQGQW AEDVLGAKTW DTETEDLTEN GQDLRRTLTH IKDQKGGLHS LQEIRVCEIH EDSSTRGSRH FYYDGELFLS QNLETQESTV PQSSRAQTLA MNVTNFWKED AMKTKTHYRA MQADCLQKLQ RYLKSGVAIR RTVPPMVNVT CSEVSEGNIT VTCRASSFYP RNITLTWRQD GVSLSHNTQQ WGDVLPDGNG TYQTWVATRI RQGEEQRFTC YMEHSGNHGT HPVPSGKVLV LQSQRTD
包裝清單:
包裝
XY7149-10μg
10μg
XY7149-50μg
50μg
XY7149-100μg
100μg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復凍融。 注意事項: 由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結(jié)合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。 本產(chǎn)品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請穿實驗服并戴一次性手套操作。