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- 產(chǎn)品名稱：Recombinant Human SPARC, His-tag
- 產(chǎn)品型號(hào)：
- 產(chǎn)品展商：XYbscience
- 產(chǎn)品文檔：無(wú)相關(guān)文檔
簡(jiǎn)單介紹
Recombinant Human SPARC, His-tag由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)???因子或重組蛋白不會(huì)粘附于管壁。Recombinant Human SPARC, His-tag所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
產(chǎn)品描述
Recombinant Human SPARC, His-tag
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY7167-1mg
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Recombinant
Human SPARC, His-tag
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1mg
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7722.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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6678
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P09486
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E. coli
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314aa
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36.1kDa
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His
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About this
Antibody
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Name
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Recombinant
Human SPARC, His-tag (Recombinant Human Secreted Protein Acidic and Rich in
Cysteine, His-tag; rHuSPARC, His-tag)；重組人富含半胱氨酸酸性分泌蛋白，His標(biāo)簽
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Synonyms
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BM-40;
Osteonectin; Basement-membrane protein 40; BM-40; ONcysteine-rich protein;
osteonectin; Osteonectin; Secreted protein acidic and rich in cysteine;
secreted protein, acidic, cysteine-rich (osteonectin)
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by its
ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is
less than 3.0μg/ml, corresponding to a specific activity of
>333IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rHuSPARC, His-tag as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Others
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Background
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Secreted
protein acidic and rich in cysteine (SPARC), also named as osteonectin or
BM-40, is an acronym for "secreted protein, acidic and rich in
cysteine". It is encoded by the SPARC gene in humans. The protein is a
glycoprotein of 40kDa, (303 amino acid residues) and consists of 17a.a.
signal sequence, an N-terminal acidic region that binds calcium, a
follistatin domain containing Kazal-like sequences, and a C-terminal
extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is
the founding member of a family of secreted matricellular proteins with
similar domain structure. It is produced by fibroblasts, capillary
endothelial cells, platelets and macrophages, especially in areas of tissue
morphogenesis and remodeling. SPARC is required for the collagen in bone to
become calcified but is also involved in extracellular matrix synthesis and
promotion of changes to cell shape. The gene product has been associated with
tumor suppression but has also been correlated with metastasis based on changes
to cell shape which can promote tumor cell invasion.
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Amino Acid
Sequence
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MSYYHHHHHH DYDIPTTENL YFQGAMGSAP
QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN
TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE
LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF
PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK
DLVI
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包裝清單:
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY7167-10μg
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Recombinant
Human SPARC, His-tag
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10μg
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XY7167-50μg
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Recombinant
Human SPARC, His-tag
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50μg
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XY7167-100μg
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Recombinant
Human SPARC, His-tag
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100μg
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XY7167-1mg
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Recombinant
Human SPARC, His-tag
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1mg
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-
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說(shuō)明書
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1份
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保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。
注意事項(xiàng):
由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡(jiǎn)介中Reconstitution一欄的信息配制儲(chǔ)存液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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