Recombinant Human SHH
產(chǎn)品編號(hào)
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價(jià)格
XY7212-1mg
1mg
14411.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
6469
Q15465
E. coli
176aa
19.8kDa
－
About this Antibody
Name
Recombinant Human SHH (Recombinant Human Sonic Hedgehog Protein N-Terminus; rHuSHH)；重組人音猬因子
Synonyms
Shh; HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog; sonic hedgehog homolog; sonic hedgehog protein; TPT; TPTPS
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1μg/ml, corresponding to a specific activity of >1.0×103IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 150mM NaCl.
Endotoxin
Less than 1EU/μg of rHuSHH as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
Sonic Hedgehog (SHH) is one of three proteins of the Hedgehog (Hh) family, which also contains Desert Hedgehog (DHH) and Indian Hedgehog (IHH). The three members share a high degree of amino-acid sequence identity (e.g., SHH and IHH are 93% identical). SHH is expressed in fetal intestine, liver, lung, and kidney, but not in ***** tissues. The protein consists of 462a.a. with a 23a.a. signal peptide at N-terminus, and is further cleaved into SHH N-Terminus and C-Terminus. SHH has the most critical roles in development, acting as a morphogen involved in patterning many systems, including the limb and midline structures in the brain, spinal cord, the thalamus by the zona limitans intrathalamica and the teeth. In the absence of Sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. SHH-N retains all known signaling capabilities, and can be lipid-modified without receptor affinity reducing, but has more potent than the unmodified form. The rHuSHH has an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
Amino Acid Sequence
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
包裝清單:
包裝
XY7212-5μg
5μg
XY7212-25μg
25μg
XY7212-100μg
100μg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請(qǐng)根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。 注意事項(xiàng): 由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲(chǔ)存液。 本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。