Recombinant Human Vimentin
產(chǎn)品編號(hào)
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價(jià)格
XY7262-1mg
1mg
5665.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
7431
P08670
E. coli
465aa
53.5kDa
－
About this protein
Name
Recombinant Human Vimentin (Recombinant Human Vimentin; rHuVimentin)；重組人波形蛋白
Synonyms
VIM; FLJ36605; vimentin
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Data not available.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in 30% Acetonitrile and 0.1% TFA.
Endotoxin
Less than 0.1EU/μg of rHuVimentin as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
Vimentin is a type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells, so it is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. Vimentin is a homopolymer assembled from elementary dimers which is mediated by the central alpha-helical coiled-coil rod region. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol and it is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Also, vimentin is found to control the transport of low-density lipoprotein, LDL, -derived cholesterol from alysosome to the site of esterification. Recombinant human vimentin contains 465 amino acid residues and it shares 97%a.a. sequence identity with murine and rat vimentin.
Amino Acid Sequence
STRSVSSSSY RRMFGGPGTA SRPSSSRSYV TTSTRTYSLG SALRPSTSRS LYASSPGGVY ATRSSAVRLR SSVPGVRLLQ DSVDFSLADA INTEFKNTRT NEKVELQELN DRFANYIDKV RFLEQQNKIL LAELEQLKGQ GKSRLGDLYE EEMRELRRQV DQLTNDKARV EVERDNLAED IMRLREKLQE EMLQREEAEN TLQSFRQDVD NASLARLDLE RKVESLQEEI AFLKKLHEEE IQELQAQIQE QHVQIDVDVS KPDLTAALRD VRQQYESVAA KNLQEAEEWY KSKFADLSEA ANRNNDALRQ AKQESTEYRR QVQSLTCEVD ALKGTNESLE RQMREMEENF AVEAANYQDT IGRLQDEIQN MKEEMARHLR EYQDLLNVKM ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVINETSQH HDDLE
包裝清單:
包裝
XY7262-20μg
20μg
XY7262-100μg
100μg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會(huì)引起部分失活，所以**配制成相應(yīng)濃度的儲(chǔ)存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲(chǔ)存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。 注意事項(xiàng): 由于有些塑料管壁對(duì)某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會(huì)粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲(chǔ)存液。 本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。