Choline Kinase (Crude Enzyme)

       Choline Kinase，中文名為膽堿激酶，以三磷酸腺苷(ATP)作為磷酸的供體，催化膽堿發(fā)生磷酸化。本產(chǎn)品為大腸桿菌表達(dá)的膽堿激酶，并經(jīng)簡(jiǎn)單純化獲得的可以確保達(dá)到一定酶活力的粗酶液。
       由于本產(chǎn)品需新鮮制備，確認(rèn)訂購后約6個(gè)工作日才可以發(fā)貨。
       本產(chǎn)品可以提供十公斤、百公斤乃至噸級(jí)的粗酶液。
       Choline kinase (also known as CK,ChoK and choline phosphokinase) is an enzyme which catalyzes the first
reaction in the choline pathway for phosphatidylcholine (PC) biosynthesis. This reaction involves the transfer of a phosphate group from adenosine triphosphate (ATP) to choline in order to form phosphocholine. Thus, the two substrates of this enzyme are ATP and choline, whereas its two products are adenosine diphosphate (ADP) and O-phosphocholine. Choline kinase requires magnesium ions (+2) as a cofactor for this reaction. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The first detailed investigation of the enzyme was conducted by McCamen in 1962, where it was shown that the brain is the richest source of the enzyme in mammalian tissue. A related enzyme, ethanolamine kinase, tends to co-purify with choline kinase leading to a suggestion that the two activities are mediated by two distinct active sites on a single protein. These enzymes participate in glycine, serine and threonine metabolism and glycerophospholipid metabolism. In mammalian cells, the enzyme exists as three isoforms: CKα-1,CKα-2 and CKβ. These isoforms are encoded by two separate genes, CHKA and CHKB and are only active in their homodimeric, heterodimeric and oligomeric forms. This product with the indicated enzyme activity was briefly purified from engineered E.coli.
產(chǎn)品詳細(xì)信息見下表:

包裝清單：

保存條件：
       -20℃或更低溫度保存，至少1個(gè)月有效。
注意事項(xiàng)：
       由于本產(chǎn)品需新鮮制備，在您確認(rèn)訂購后約6個(gè)工作日才能發(fā)貨。
       粗酶液的每次凍融均可能會(huì)引起部分失活，所以收到酶液后請(qǐng)根據(jù)需要進(jìn)行分裝，并置于-20℃或更低溫度保存。
       隨著保存時(shí)間的延長(zhǎng)，酶活力會(huì)有一定程度的下降，收到產(chǎn)品后應(yīng)盡快使用。
       本產(chǎn)品在生產(chǎn)和保存的過程中可能會(huì)有混濁或沉淀產(chǎn)生，融化后混勻即可，不影響正常使用。
       本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
       為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

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