Glutathione Peroxidase 1 Mouse Monoclonal Antibody
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY0162
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Glutathione
Peroxidase 1 Mouse Monoclonal Antibody
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50μl
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416.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Mouse
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WB, FC, IHC,
ICC, IF
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H
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22KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000
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-
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1:200
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1:200
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1:200
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1:100-1:200
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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Glutathione
Peroxidase 1 Mouse Monoclonal Antibody
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Category
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Monoclonal
antibody(mAb); Primary antibody
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Isotype
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IgG2b
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Purification
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ProA XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing mice with a recombinant protein corresponding to a
region of GPX1.
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Gene ID
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2876
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SwissProt
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P07203
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Synonyms
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GPX1; GSHPX1;
MGC14399; MGC88245
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Category
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Metabolism
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Background
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GPX1 is
ubiquitously expressed in many tissues, where it protects cells from
oxidative stress. Within cells, it localizes to the cytoplasm and
mitochondria. As a glutathione peroxidase, GPx1 functions in the
detoxification of hydrogen peroxide, specifically by catalyzing the reduction
of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the
reduction of other organic hydroperoxides, such as lipid peroxides, to the
corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant,
but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead. The protein
encoded by this gene protects from CD95-induced apoptosis in cultured breast
cancer cells and inhibits 5-lipoxygenase in blood cells, and its
overexpression delays endothelial cell death and increases resistance to
toxic challenges, especially oxidative stress. This protein is one of only a
few proteins known in higher vertebrates to contain selenocysteine, which occurs
at the active site of glutathione peroxidase and is coded by the nonsense
(stop) codon TGA.
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包裝清單：
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY0162
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Glutathione
Peroxidase 1 Mouse Monoclonal Antibody
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50μl
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AZ050
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Western一抗稀釋液
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50ml
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—
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說明書
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1份
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保存條件：
Glutathione
Peroxidase 1 Mouse Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng)：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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