HER2/ErbB2 Rabbit Polyclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY0177
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HER2/ErbB2
Rabbit Polyclonal Antibody
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50μl
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416.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, ICC, IHC,
IF
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H
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140KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:500-1:1000
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-
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1:200
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1:200
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1:200
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-
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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HER2/ErbB2
Rabbit Polyclonal Antibody
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Category
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Polyclonal
antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to human ErbB2.
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Gene ID
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2064
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SwissProt
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P04626
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Synonyms
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CerbB2
antibody; CerbB2/neu protein antibody; ErbB2 antibody; HER2 antibody
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Category
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RTK
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Background
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HER2 is a
member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family.
Amplification or overexpression of this oncogene has been shown to play an
important role in the development and progression of certain aggressive types
of breast cancer. In recent years the protein has become an important
biomarker and target of therapy for approx. 30% of breast cancer patients.
Over-expression is also known to occur in ovarian, stomach, and aggressive
forms of uterine cancer, such as uterine serous endometrial carcinoma. The
expression of HER2 is regulated by signaling through estrogen receptors.
Normally, estradiol and tamoxifen acting through the estrogen receptor
down-regulate the expression of HER2. However, when the ratio of the
coactivator AIB-3 exceeds that of the corepressor PAX2, the expression of
HER2 is upregulated in the presence of tamoxifen, leading to
tamoxifen-resistant breast cancer.
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包裝清單：
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY0177
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HER2/ErbB2
Rabbit Polyclonal Antibody
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50μl
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AZ050
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Western一抗稀釋液
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50ml
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—
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說明書
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1份
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保存條件：
HER2 / ErbB2
Rabbit Polyclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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