HSP60 Mouse Monoclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY0186
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HSP60 Mouse
Monoclonal Antibody
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50μl
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416.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Mouse
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WB, IHC, ICC,
IF
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H, M, R
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61KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000
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-
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1:100
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1:100
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1:100
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-
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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HSP60 Mouse
Monoclonal Antibody
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Category
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Monoclonal
antibody(mAb); Primary antibody
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Isotype
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IgG1
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Purification
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ProA XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing mice with a synthetic peptide (KLH-coupled)
corresponding to HSP60.
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Gene ID
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3329
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SwissProt
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P10809
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Synonyms
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CPN60;
CHAPERONIN 60-KD; GroEL E. COLI HOMOLOG OF HEAT-SHOCK 60-KD PROTEIN1; heat
shock 60kDa protein 1 (chaperonin)
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Category
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Protein
Folding and TrXYficking
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Background
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The 60 kDa
heat shock protein (HSP60) is a highly conserved protein that acts as a
chaperone involved in correct folding of newly synthesized proteins. Mainly
expressed in mitochondria, HPS60 has also been found in the cytosol of normal
heart and muscle cells. The HSP60 proteins are abundant in the cell and are
highly conserved between bacteria and human, with 50%-60% sequence homology.
Mammalian HSP60 is expressed in the mitochondria at a low level and rapidly
up-regulated under stresses, such as heat shock, and on some occasions with
changes in intracellular location including expression on the cell surface.
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包裝清單：
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY0186
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HSP60 Mouse
Monoclonal Antibody
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50μl
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AZ050
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Western一抗稀釋液
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50ml
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—
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說明書
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1份
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保存條件：
HSP60 Mouse
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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