HSP70 Rabbit Polyclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY0189
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HSP70 Rabbit
Polyclonal Antibody
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50μl
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416.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IHC, ICC,
IF
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H, M, R
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70KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:500-1:1000
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-
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1:200
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1:200
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1:200
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-
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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HSP70 Rabbit
Polyclonal Antibody
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Category
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Polyclonal
antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to HSP70.
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Gene ID
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3304
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SwissProt
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P08107
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Synonyms
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HSP72; HSPA1;
HSP70I; HSP70-1; HSP70-1A;HSPA1A
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Category
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Protein
Folding and TrXYficking
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Background
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The 70
kilodalton heat shock proteins (Hsp70s) are a family of conserved
ubiquitously expressedheat shock proteins. Proteins with similar structure
exist in virtually all living organisms. The Hsp70s are an important part of
the cell's machinery for protein folding, and help to protect cells from
stress. When not interacting with a substrate peptide, Hsp70 is usually in an
ATP bound state. Hsp70 by itself is characterized by a very weak ATPase
activity, such that spontaneous hydrolysis will not occur for many minutes.
As newly synthesized proteins emerge from the ribosomes, the substrate
binding domain of Hsp70 recognizes sequences of hydrophobic amino acid
residues, and interacts with them. This spontaneous interaction is
reversible, and in the ATP bound state Hsp70 may relatively freely bind and
release peptides. However, the presence of a peptide in the binding domain
stimulates the ATPase activity of Hsp70, increasing its normally slow rate of
ATP hydrolysis.
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包裝清單：
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY0189
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HSP70 Rabbit
Polyclonal Antibody
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50μl
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AZ050
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Western一抗稀釋液
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50ml
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—
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說明書
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1份
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保存條件：
HSP70 Rabbit
Polyclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
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