Isocitrate dehydrogenase/IDH1 Mouse Monoclonal Antibody
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
產(chǎn)品包裝
|
產(chǎn)品價格
|
|
XY0210
|
Isocitrate
dehydrogenase/IDH1 Mouse Monoclonal Antibody
|
50μl
|
416.00元
|
|
來源
|
用途
|
交叉反應性
|
分子量
|
|
Mouse
|
WB, IHC
|
H
|
47KDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1000
|
-
|
-
|
1:100-1:200
|
-
|
-
|
-
|
-
|
抗體詳細信息如下:
|
About this
Antibody
|
|
Name
|
Isocitrate
dehydrogenase/IDH1 Mouse Monoclonal Antibody
|
|
Category
|
Monoclonal
antibody(mAb); Primary antibody
|
|
Isotype
|
IgG1
|
|
Purification
|
ProA XYfinity
purified
|
|
About the
Immunogen
|
|
Immunogen
|
This antibody
is produced by immunizing mice with a recombinant protein corresponding to a
region of IDH1.
|
|
Gene ID
|
3417
|
|
SwissProt
|
O75874
|
|
Synonyms
|
IDCD; IDH;
IDP; IDPC; PICD
|
|
Category
|
Metabolism
|
|
Background
|
Isocitrate
dehydrogenase (IDH) is an enzyme that catalyzes the oxidative decarboxylation
of isocitrate, producing alpha-ketoglutarate (α-ketoglutarate) and
CO2. In humans, IDH exists in three isoforms: IDH3 catalyzes the third step
of the citric acid cycle while converting NAD+ to NADH in the mitochondria.
The isoforms IDH1 and IDH2 catalyze the same reaction outside the context of
the citric acid cycle and use NADP+ as a cofactor instead of NAD+. They
localize to the cytosol as well as the mitochondrion and peroxisome.
Mutations in IDH1 are also implicated in cancer. Originally mutations in IDH1
were detected in an integrated genomic analysis of human glioblastoma
multiforme. In addition to being mutated in diffuse gliomas, IDH1 has also
been shown to harbor mutations in human acute myeloid leukemia (AML).
|
包裝清單：
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
XY0210
|
Isocitrate
dehydrogenase/IDH1 Mouse Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀釋液
|
50ml
|
|
—
|
說明書
|
1份
|
保存條件：
Isocitrate
dehydrogenase/IDH1 Mouse Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
- 溫馨提示：為規(guī)避購買風險，建議您在購買前務必確認供應商資質(zhì)與產(chǎn)品質(zhì)量。
- 免責申明：以上內(nèi)容為注冊會員自行發(fā)布，若信息的真實性、合法性存在爭議，平臺將會監(jiān)督協(xié)助處理，歡迎舉報