Ku70 Mouse Monoclonal Antibody
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY0213
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Ku70 Mouse
Monoclonal Antibody
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50μl
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416.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Mouse
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WB, IHC
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H, M
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70KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000
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-
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-
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1:50-1:100
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-
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-
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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Ku70 Mouse
Monoclonal Antibody
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Category
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Monoclonal
antibody(mAb); Primary antibody
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Isotype
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IgG2a
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Purification
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ProA XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing mice with a recombinant protein corresponding to a
region of Ku70.
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Gene ID
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2547
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SwissProt
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P12956
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Synonyms
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70 kDa subunit
of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA
helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit;
CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit;
CTC75; CTCBF
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Category
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Cell Cycle /
Checkpoint Control
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Background
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Together, Ku70
and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break
ends and is required for the non-homologous end joining (NHEJ) pathway of DNA
repair. It is also required for V(D)J recombination, which utilizes the NHEJ
pathway to promote antigen diversity in the mammalian immune system.In
addition to its role in NHEJ, Ku is also required for telomere length
maintenance and subtelomeric gene silencing. Ku was originally identified
when patients with systemic lupus erythematosus were found to have high
levels of autoantibodies to the protein.
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包裝清單：
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY0213
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Ku70 Mouse
Monoclonal Antibody
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50μl
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AZ050
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Western一抗稀釋液
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50ml
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—
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說明書
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1份
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保存條件：
Ku70 Mouse
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng)：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請(qǐng)注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請(qǐng)4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測(cè)效果，請(qǐng)考慮使用上述檢測(cè)專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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