MGMT Rabbit Polyclonal Antibody
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產品編號
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產品名稱
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產品包裝
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產品價格
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XY0228
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MGMT Rabbit
Polyclonal Antibody
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50μl
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416.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, FC, IHC,
ICC, IF
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H
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22KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:500-1:1000
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-
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1:200
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1:200
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1:200
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1:100
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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MGMT Rabbit
Polyclonal Antibody
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Category
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Polyclonal
antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to MGMT.
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Gene ID
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4255
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SwissProt
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P16455
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Synonyms
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Methylated-DNA-protein-cysteine
methyltransferase; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase; MGMT
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Category
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Cell Cycle /
Checkpoint Control
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Background
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MGMT
(O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that
participates in a suicide reaction that specifically removes methyl or alkyl
groups from the O (6) position of guanine, restoring guanine to its normal
form without causing DNA breaks. MGMT protects cells from alkylating toxins,
and is an important factor in drug resistance to alkylating therapeutic
agents. It is ubiquitously expressed in normal human tissues and is
overexpressed in many types of human tumors, but epigenetically silenced in
other tumors. MGMT silencing is a marker associated with poor prognosis, but
is a good predictive marker for response to alkylating agent chemotherapy.
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包裝清單：
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產品編號
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產品名稱
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包裝
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XY0228
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MGMT Rabbit
Polyclonal Antibody
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50μl
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AZ050
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Western一抗稀釋液
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50ml
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—
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說明書
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1份
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保存條件：
MGMT Rabbit
Polyclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
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