Ras Rabbit Polyclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY0273
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Ras Rabbit
Polyclonal Antibody
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50μl
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416.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, FC, IHC,
ICC, IF
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H, M, R
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21KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000
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-
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1:200
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1:200
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1:200
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1:100-1:200
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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Ras Rabbit
Polyclonal Antibody
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Category
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Polyclonal
antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to H-Ras, N-Ras and K-Ras.
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Gene ID
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4893/3265/3845
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SwissProt
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P01111/P01112/P01116
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Synonyms
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GTPase NRas;
Transforming protein N-Ras; NRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras;
Transforming protein p21; c-H-ras; p21ras; HRAS; HRAS1; GTPase KRas; K-Ras 2;
Ki-Ras; c-K-ras; c-Ki-ras; KRAS; KRAS2; RASK2
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Category
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-
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Background
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Ras
superfamily is a protein superfamily of small GTPases, which are all related,
to a degree, to the Ras protein subfamily (the key human members of which are
KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled
receptors activate Ras, which then stimulates the RXY-MEK-MAPK pathway.
GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras.
However, research studies have shown that in 30% of human tumors, point
mutations in Ras prevent the GAP-mediated inhibition of this pathway. The
most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D),
which prevents Ras inactivation, possibly by increasing the overall rigidity
of the protein. This antibody is predicted to react with H-Ras, N-Ras and
K-Ras.
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包裝清單：
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY0273
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Ras Rabbit
Polyclonal Antibody
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50μl
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AZ050
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Western一抗稀釋液
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50ml
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—
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說明書
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1份
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保存條件：
Ras Rabbit
Polyclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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