Sequestosome-1 Mouse Monoclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY0279
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Sequestosome-1
Mouse Monoclonal Antibody
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50μl
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416.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Mouse
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WB, FC, IHC,
ICC, IF
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H, M, R
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62KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:500-1:1000
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-
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1:200
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1:200
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1:200
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1:50-1:100
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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Sequestosome-1
Mouse Monoclonal Antibody
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Category
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Monoclonal
antibody(mAb); Primary antibody
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Isotype
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IgG1
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Purification
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ProA XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing mice with a synthetic peptide (KLH-coupled)
corresponding to SQSTM1.
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Gene ID
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8878
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SwissProt
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Q13501
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Synonyms
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OSIL antibody;
Oxidative stress induced like antibody p60 antibody p62 antibody p62B
antibody Paget disease of bone 3 antibody PDB 3 antibody PDB3 antibody
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Category
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Autophagy
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Background
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Sequestosome 1
(SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling,
oxidative stress and autophagy. It was first identified as a protein that
binds to the SH2 domain of p56Lck and independently found to interact with
PKCζ. SQSTM1 was subsequently found to interact with ubiquitin,
providing a scXYfold for several signaling proteins and triggering
degradation of proteins through the proteasome or lysosome. Interaction
between SQSTM1 and TRXY6 leads to the K63-linked polyubiquitination of TRXY6
and subsequent activation of the NF-κB pathway. It
may play a role in titin/TTN downstream signaling in muscle cells and
regulate signaling cascades through ubiquitination. P62 may be involved in
cell differentiation, apoptosis, immune response and regulation of K+
channels.
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包裝清單：
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY0279
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Sequestosome-1
Mouse Monoclonal Antibody
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50μl
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AZ050
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Western一抗稀釋液
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50ml
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—
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說明書
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1份
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保存條件：
Sequestosome-1
Mouse Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng)：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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