TOP2A Rabbit Polyclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY0303
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TOP2A Rabbit
Polyclonal Antibody
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50μl
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416.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, IHC, ICC,
FC, IF
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H, M, R
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174KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:500-1:1000
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-
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1:200
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1:200
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1:200
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1:100-1:200
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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TOP2A Rabbit
Polyclonal Antibody
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Category
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Polyclonal
antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to TOP2A.
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Gene ID
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7153
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SwissProt
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P11388
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Synonyms
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DNA
topoisomerase II; DNA topoisomerase II-alpha; EC 5.99.1.3, P11388-1; TOP-2;
TOP2; TOP2A; TP2A; alpha isozyme
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Category
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Chromatin
Regulation / Nuclear Function
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Background
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This gene
encodes a DNA topoisomerase, an enzyme that controls and alters the topologic
states of DNA during transcription. This nuclear enzyme is involved in
processes such as chromosome condensation, chromatid separation, and the
relief of torsional stress that occurs during DNA transcription and
replication. It catalyzes the transient breaking and rejoining of two strands
of duplex DNA which allows the strands to pass through one another, thus
altering the topology of DNA. Two forms of this enzyme exist as likely
products of a gene duplication event. It is essential during mitosis and
meiosis for proper segregation of daughter chromosomes.
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包裝清單：
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY0303
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TOP2A Rabbit
Polyclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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—
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說明書
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1份
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保存條件：
TOP2A Rabbit
Polyclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項：
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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