Cytokeratin 19 Rabbit Monoclonal Antibody
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
產(chǎn)品包裝
|
產(chǎn)品價格
|
|
XY1006
|
Cytokeratin 19
Rabbit Monoclonal Antibody
|
50μl
|
618.00元
|
|
來源
|
用途
|
交叉反應(yīng)性
|
分子量
|
|
Rabbit
|
WB, IP, IF,
IHC, ICC, FC
|
H, M
|
40.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:2,000
|
1:10-1:40
|
1:10-1:40
|
1:10-1:40
|
1:10-1:40
|
1:2-1:20
|
-
|
-
|
抗體詳細信息如下:
|
About this
Antibody
|
|
Name
|
Cytokeratin 19
Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
3880(Human);
16669(Mouse)
|
|
SwissProt
|
P08727(Human);
P19001(Mouse)
|
|
Synonyms
|
K19; CK19;
K1CS; KRT19
|
|
Category
|
Cytoskeletal
Signaling
|
|
Background
|
Cytokeratins
comprise a diverse group of intermediate filament proteins (IFPs) that are
expressed as pairs in both keratinized and non-keratinized epithelial tissue.
Cytokeratins play a critical role in differentiation and tissue
specialization and function to maintain the overall structural integrity of
epithelial cells and have been found to be useful markers of tissue
differentiation, which is directly applicable to the characterization of
malignant tumors. For example, many types of cancer cells express Cytokeratin
19 (CK19), an epithelial cytoskeletal protein within the suprabasal squamous
epithelium. Cytokeratin 19 is a specific marker of moderate to severe
dysplasia and carcinoma in situ in oral cavity squamous epithelium, and
measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma.
Cytokeratin 19 fragment levels in serum have been documented as a marker for
lung cancer. Clinical investigations have suggested that serum CYFRA 21-1, a
fragment of Cytokeratin 19, may be among the most useful tumor markers.
|
包裝清單:
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
XY1006
|
Cytokeratin 19
Rabbit Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說明書
|
1份
|
保存條件:
Cytokeratin 19
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(xué)(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
- 溫馨提示：為規(guī)避購買風(fēng)險，建議您在購買前務(wù)必確認供應(yīng)商資質(zhì)與產(chǎn)品質(zhì)量。
- 免責(zé)申明：以上內(nèi)容為注冊會員自行發(fā)布，若信息的真實性、合法性存在爭議，平臺將會監(jiān)督協(xié)助處理，歡迎舉報