p75 NGF Receptor Rabbit Monoclonal Antibody
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY1033
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p75 NGF
Receptor Rabbit Monoclonal Antibody
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50μl
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618.00元
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來(lái)源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC
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H, M, R
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75.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000-1:5000
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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p75 NGF
Receptor Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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4804(Human);
24596(Mouse)
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SwissProt
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P08138(Human);
Q9Z0W1(Mouse); P07174(Rat)
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Synonyms
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CD271; p75NTR;
TNFRSF16; p75(NTR); Gp80-LNGFR; NGFR
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Category
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Cell Cycle
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Background
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The Trk
oncogene encodes a membrane-spanning protein tyrosine kinase, gp140Trk, whose
expression is restricted in vivo to neurons of the sensory spinal and cranial
ganglia of neural crest origin. Nerve growth factor (NGF) stimulates tyrosine
phosphorylation of Trk A in neural cell lines and in embryonic dorsal root
ganglia. Tyrosine phosphorylation of Trk by NGF is rapid, specific and occurs
with picomolar quantities of factor, indicating that the response is mediated
by physiological amounts of NGF, suggesting that Trk A participates in the
primary signal transduction mechanism of NGF. An additional component of the
Trk A receptor complex, NGFR p75, binds to the neurotrophic factors with low XYfinity
but is required for efficient signaling. NGFR p75 accelerates Trk A
activation and may recruit downstream effector molecules to the liganded
complex.
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包裝清單:
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產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY1033
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p75 NGF
Receptor Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說(shuō)明書(shū)
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1份
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保存條件:
p75 NGF Receptor Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長(zhǎng)期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請(qǐng)注意回收使用過(guò)的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過(guò)的稀釋抗體，請(qǐng)4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測(cè)效果，請(qǐng)考慮使用上述檢測(cè)專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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