JNK1+JNK2+JNK3 Rabbit Monoclonal Antibody
產(chǎn)品編號(hào)
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY1048
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JNK1+JNK2+JNK3
Rabbit Monoclonal Antibody
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50μl
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618.00元
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來(lái)源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IP, IF,
ICC
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H, M, R,Cw,
Mk, Z
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46/54 kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液,可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整):
WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000-1:5000
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1:50-1:200
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1:50-1:200
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-
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1:50-1:200
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-
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-
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-
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抗體詳細(xì)信息如下:
About this
Antibody
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Name
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JNK1+JNK2+JNK3
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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5599/5601/5602(Human);
26414/26419/26420(Mouse); 116554/50658/25272(Rat)
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SwissProt
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P45983/P45984/P53779(Human);
Q61831/Q91Y86/Q9WTU6 (Mouse); P49185/P49186/P49187(Rat)
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Synonyms
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C Jun kinase
2; c Jun N terminal kinase 1; c Jun N terminal kinase 2; c Jun N terminal
kinase 3; c-Jun N-terminal kinase 1; JNK 46; JNK 55; JNK; MAP kinase 10; MAP
kinase 9; MAP kinase 8;Mitogen activated protein kinase 10; Mitogen activated
protein kinase 9; Mitogen activated protein kinase 8; PRKM9; PRKM8; PRKM10
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Category
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MAPK
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Background
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c-Jun
N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity
of c-Jun. JNKs originate from three genes that yield 10 isoforms through
alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and
JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2,
and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses
to stress and influence regulation of cell growth and transformation. The
human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares
83% amino acid identity with JNK2. JNK1 is necessary for normal activation
and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector
cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast
epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos,
c-Jun, and other signaling kinases, including MEKK1 and p38.
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包裝清單:
產(chǎn)品編號(hào)
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產(chǎn)品名稱
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包裝
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XY1048
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JNK1+JNK2+JNK3
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說(shuō)明書
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1份
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保存條件:
JNK1+JNK2+JNK3 Rabbit Monoclonal Antibody -20℃保存,Western一抗稀釋液-20℃或4℃保存,一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存,長(zhǎng)期不使用可以考慮-20℃保存,但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn),請(qǐng)注意回收使用過(guò)的稀釋抗體?;厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體,包括已經(jīng)使用過(guò)的稀釋抗體,請(qǐng)4℃保存。
回收后重復(fù)使用的抗體,使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象,可以10,000g離心1-3分鐘,取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況,則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測(cè)效果,請(qǐng)考慮使用上述檢測(cè)專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用,不得用于臨床診斷或**,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
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