PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody
產(chǎn)品編號(hào) |
產(chǎn)品名稱 |
產(chǎn)品包裝 |
產(chǎn)品價(jià)格 |
XY1114 |
PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody |
50μl |
618.00元 |
來源 |
用途 |
交叉反應(yīng)性 |
分子量 |
Rabbit |
WB, IP, IF, IHC, ICC |
H, M, R |
54.0kDa |
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液,可以用于Western檢測或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整):
WB |
IP |
IF |
IHC |
ICC |
FC |
ELISA |
ChIP |
1:1000-1:10000 |
1:50-1:200 |
1:50-1:200 |
1:200-1:500 |
1:50-1:200 |
- |
- |
- |
抗體詳細(xì)信息如下:
About this Antibody |
|
Name |
PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody |
Category |
Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
Isotype |
IgG |
Purification |
XYfinity purification |
About the Immunogen |
|
Immunogen |
Recombinant protein |
Gene ID |
8503(Human); 18710(Mouse); 60664(Rat) |
SwissProt |
Q92569(Human); Q64143(Mouse); Q63789(Rat) |
Synonyms |
p55; p55PIK; p55-GAMMA |
Category |
PI3K/Akt/mTOR |
Background |
Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3’ position. PI 3-kinase p55γ (PIK3R3) is comprised of a catalytic subunit and a regulatory subunit. The human p55γ protein is composed of a rare amino terminal region followed by a proline-rich motif and two Src homology 2 (SH2) domains. PI 3-kinase p55γ mRNAs are expressed in most human fetal and ***** tissues; predominant expression is observed in the ***** testis. Splice variant(s) of PI 3-kinase p55 γ have been identified; one of which has a deletion of 36 amino acids at the amino terminus and another which has an insertion of 59 amino acids at position 256 between the SH2 domains. Research suggests that PI 3-kinase p55 γ interacts with the IGFIR (Insulin-like growth factor-I receptor) and IR (Insulin receptor) and may be involved in PI 3-kinase activation by these receptors. |
包裝清單:
產(chǎn)品編號(hào) |
產(chǎn)品名稱 |
包裝 |
XY1114 |
PI 3 Kinase p55 gamma Rabbit Monoclonal Antibody |
50μl |
XY050 |
Western一抗稀釋液 |
50ml |
- |
說明書 |
1份 |
保存條件:
PI 3 Kinase p55
gamma Rabbit Monoclonal Antibody -20℃保存,Western一抗稀釋液-20℃或4℃保存,一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存,長期不使用可以考慮-20℃保存,但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn),請(qǐng)注意回收使用過的稀釋抗體?;厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體,包括已經(jīng)使用過的稀釋抗體,請(qǐng)4℃保存。
回收后重復(fù)使用的抗體,使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象,可以10,000g離心1-3分鐘,取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況,則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果,請(qǐng)考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用,不得用于臨床診斷或**,不得用于食品或藥品,不得存放于普通住宅內(nèi)。
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