PTEN Rabbit Monoclonal Antibody
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
產(chǎn)品包裝
|
產(chǎn)品價(jià)格
|
|
XY1426
|
PTEN Rabbit
Monoclonal Antibody
|
50μl
|
618.00元
|
|
來源
|
用途
|
交叉反應(yīng)性
|
分子量
|
|
Rabbit
|
WB, IF, IHC,
ICC
|
H, M, R
|
54.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:5,000
|
-
|
1:50-1:500
|
1:50-1:200
|
1:50-1:500
|
-
|
-
|
-
|
抗體詳細(xì)信息如下:
|
About this
Antibody
|
|
Name
|
PTEN Rabbit
Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
5728(Human);
19211(Mouse); 50557(Rat)
|
|
SwissProt
|
P60484(Human);
O08586(Mouse); O54857(Rat)
|
|
Synonyms
|
10q23del; BZS;
CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1
|
|
Category
|
PI3K/Akt/mTOR
|
|
Background
|
As human
tumors progress to advanced stages, one genetic alteration that occurs at
high frequency is a loss of heterozygosity (LOH) at chromosome 10q23. Mapping
of homozygous deletions on this chromosome led to the isolation of the PTEN
gene, also designated MMAC1 (for mutated in multiple advanced cancers) and
TEP1. This candidate tumor suppressor gene exhibits a high frequency of
mutations in human glioblastomas and is also mutated in other cancers,
including sporadic brain, breast, kidney and prostate cancers. PTEN has been
associated with Cowden disease, an autosomal dominant cancer predisposition
syndrome. The PTEN gene product is a putative protein tyrosine phosphatase
that is localized to the cytoplasm and shares extensive homology with the
cytoskeletal proteins tensin and auxilin. Gene transfer studies have
indicated that the phosphatase domain of PTEN is essential for growth
suppression of glioma cells.
|
包裝清單:
|
產(chǎn)品編號
|
產(chǎn)品名稱
|
包裝
|
|
XY1426
|
PTEN Rabbit
Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說明書
|
1份
|
保存條件:
PTEN Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。
- 溫馨提示：為規(guī)避購買風(fēng)險(xiǎn)，建議您在購買前務(wù)必確認(rèn)供應(yīng)商資質(zhì)與產(chǎn)品質(zhì)量。
- 免責(zé)申明：以上內(nèi)容為注冊會員自行發(fā)布，若信息的真實(shí)性、合法性存在爭議，平臺將會監(jiān)督協(xié)助處理，歡迎舉報(bào)